In terms of the use of Transglutaminase, different proteins are used in the TG mix as added connective protein to enhance the overall binding action. When TG is mixed in a solvent before application, different solvents will provide different solubility which may concern operators.
For example, TG containing stromal proteins such as collagen which shows low solubility in a neutral aqueous solvent such as water but high solubility in a curing brine solution with phosphates and salts on account of the high ionic charge of this solution.
From Yu, T.Y., et al, 2017.
The solubility of different proteins under various ionic strengths further informs us of the importance of salt and phosphates in solubilizing myofibril protein. Mixing the TG into a small brine solution has in my experience the best results.
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